A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Mitochondria are sequestered inside Rab5+ endosomes after FCCP treatment in WT and Atg5-/- MEFs. Wild-type (WT) and Atg5-/- mouse embryonic fibroblasts (MEFs) were transfected with GFP-Rab5, nanobody against GFP (VHH)-APEX2, HA-Parkin, and mPlum-mito-3 using Fugene 6. Cells were treated with DMSO or 25 uM FCCP for 4 hours