In [13]: I1 = io.imread('Lenna.bmp') In [14]: I2 = io.imread('Mandrill.bmp') In [15]: I3 = io.imread('Parrots.bmp') In [16]: io.imsave('output.tiff', [I1, I2, I3]) In [17]: I4 = io.imread('output.tif') In [18]: I4.shape Out[18]: (3, 256, 256, 3) In [19]: io.imshow(I4[0]) In [20]: io.imshow(I4[1]) In [21]: io.imshow(I4[2])