Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression genome-wide in a single assay. The volume and complexity of data from RNA-seq experiments necessitate scalable, fast and mathematically principled analysis software. TopHat and Cufflinks are free, open-source software tools for gene discovery and comprehensive expression an
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Improving genetic diagnosis in Mendelian disease with transcriptome sequencing – a walk through
