Illumina/Solexa Genome Analyzer Primer/Adapter Sequences - SEQanswers
Very useful! I refined these sequences. Hopes they're clearer. Genomic DNA Adapter: 5' -------------------- -----ACACTCTTTCCCTAC ACGACGCTCTTCCGATCT (-) -------------------- -------------- 3' 3' -------------------- -----TGTGAGAAAGGGATG TGCTGCGAGAAGGCTAGp (-) -------------------- -------------- 5' Adapter: 5' -------------------- -------------------- ------------------ (-) pGATCGGAAGAGCTCGTATG CCGT
Removing duplicates is it really necessary? - SEQanswers
"Also how do you define a duplicate?" As you suggest, this can be done in at least two ways. Both have caveats. If you define duplicates as reads with identical sequence you may underestimate the presence of duplicates in your library. Read errors present in your library will result in reads with apparently different sequences that could have actually come from PCR amplifications of the same cDNA
Browsers/Viewers for RNAseq - SEQanswers
Hello everyone! I am here collecting the information of browsers/viewers especially for RNA sequencing data, which can mainly show the gene annotation track, read track (including spliced reads), and some other annotation tracks. This is the basic requirment of RNA-seq data viewing. Thanks to the Seqanswers wiki page, I listed the browsers/viewers for RNA-seq below. But due to my limited experienc
Software packages for next gen sequence analysis - SEQanswers
28 Dec 2009: This thread has been closed. Please see our wiki software portal for information about each of these packages. A reasonably thorough table of next-gen-seq software available in the commercial and public domain Integrated solutions * CLCbio Genomics Workbench - de novo and reference assembly of Sanger, Roche FLX, Illumina, Helicos, and SOLiD data. Commercial next-gen-seq software that
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SAM flag idioms - SEQanswers
Base intensities - SEQanswers
Convert BAM file to FASTQ - SEQanswers
Paired end solexa (goat/gerald) pipeline - SEQanswers
The NEXTflex™ PCR-Free DNA Sequencing Kit Reduces Bias in NGS Library Prep - SEQanswers
nextera - SEQanswers
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1000 Genomes Data/ Exon targetted - SEQanswers
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Sequencing Technologies/Companies - SEQanswers